Figure 4.

Vaccine induces MUC1-specific CD8⁺ T cells. MHC class I H2K^b DimerX (BD DimerX) was incubated overnight with either the MUC1–8mer peptide (SAPDTRPA) or glycopeptide MUC1–10mer (SAPD(T)RPAPG). Isolated spleen cells from vaccinated MUC1.tg mouse that received one cycle of DSS were stained for expression of CD3, CD4 and CD8, mixed with peptide-loaded DimerX and analyzed by flow cytometry. (A) Gated on the small CD3⁺CD8⁺ lymphocyte population (circle). (B) Gated on the CD3⁺CD8⁺ blasting lymphocyte population (square).