Figure 6. MUC1 vaccine does not induce autoimmunity. (A) Representative H&E stained colon tissue sections from vaccinated MUC1.tg and Wt mice. (B) Representative immunostaining of colon tissue sections from vaccinated MUC1.tg mice. HMPV antibody stains all MUC1; 4H5 antibody stains hypoglycosylated MUC1. Scale bars are 100 µm. (C) Isolated spleen cells from vaccinated MUC1.tg and Wt mice were stained for expression of CD3, CD4, CD8, Gr1, CD11b. Representative dot plots show percentage of CD4⁺ and CD8⁺ T cells (left panel) from the gated CD3⁺ cells and the percentage of Gr1⁺CD11b⁺ MDSC cells (right panel) from the gated non-CD3⁺ cells.