The Gal4-Tra1 interaction occurs only in galactose medium. (A) Fluorescence emission spectra in Gal4-ECFP/Tra1-EYFP cells at various time points following a shift from raffinose to galactose medium. (B) Kinetics of the Gal4-Tra1 interaction, monitored by FRET (data from A), and transcriptional induction of GAL1, monitored by primer-extension (Bhaumik and Green 2002; inset), following a shift from raffinose to galactose medium. (C) Fluorescence emission spectra in Gal4-ECFP/Gal80-EYFP cells in dextrose, raffinose, and galactose media.