Table 1.
YS- and P-Sp–derived T-progenitor cells reconstitute thymus and spleen after injection
| No. | Thymus reconstitution, n/N | SPL reconstitution, n/N | CD45.2 in SPL, %, mean ± SD | |
|---|---|---|---|---|
| WT YS | ||||
| 2 wk | 10 | 8/10 | 8/10 | 2.26 ± 1.4 |
| 14 wk | 2 | 0/3 | 2/3 | 15.5 ± 13.0 |
| WT PSP | ||||
| 2 wk | 11 | 9/11 | 9/11 | 2.61 ± 1.5 |
| 14 wk | 3 | 0/3 | 3/3 | 28.9 ± 35.8 |
| NCX1−/− YS | ||||
| 2 wk | 3 | 2/3 | 2/3 | 2 ± 1.1 |
| 4 wk | 1 | 0/1 | 1/1 | 20.9 |
| NCX1−/− PSP | ||||
| 2 wk | 2 | 2/2 | 1/2 | 2.17 |
| 5 wk | 2 | 0/2 | 2/2 | 55.2 ± 4.5 |
| Thymus | ||||
| 2 wk | 1 | 1/1 | 1/1 | 7.58 |
| 4 wk | 1 | 0/1 | 1/1 | 46.3 |
| 32 wk | 1 | 0/1 | 1/1 | 47.5 |
YS- or P-Sp–derived T-progenitor cells produced in OP9-DL1 coculture were injected into NOG neonates. At different time points after injection, engraftment in the recipient thymus and spleen were analyzed. Donor cell type > 0.1% was considered as reconstituted.