Figure 2.

EVI1 is frequently expressed in MLL-rearranged leukemias. (A) Relative EVI1 expression of patients with different 11q23 aberrations, corresponding to MLL-AF4, MLL-AF6, MLL-AF9, MLL-AF10, MLL-ENL, and other MLL-fusions. A cutoff value of 0.1 relative to the calibrator ovarian carcinoma cell line SKOV3 was chosen to classify EVI1^pos from EVI1^neg cases.¹⁹ Per MLL translocation, the percentage (%) of EVI1^pos patients is indicated. (B) EVI1 protein expression is shown in selected patient samples with MLL-rearranged leukemias. *For the MLL-AF6 sample, we observed EVI1 protein expression at longer exposure time. A 3q26/EVI1-rearranged AML and an EVI1-negative case served as, respectively, positive and negative control for EVI1 protein expression. Actin staining shows equal protein loading. MW marker indicates molecular weight marker; marking bands at, respectively 170, 130, 95 kDa on EVI1 Western and 55, 43 kDa on actin Western blot.