Table 2.

Protein levels of 35 apoptosis-related proteins after treatment of VSMCs with 1 mM L-NAME.

Protein	Control average	1 mM L-NAME average	Change	P
Bad	50.59 ± 8.08	54.73 ± 9.86	4.15	0.637
Bax	47.65 ± 14.26	50.98 ± 13.43	3.32	0.805
Bcl-2	31.37 ± 7.47	34.32 ± 5.04	2.95	0.633
Bcl-x	17.62 ± 4.92	19.02 ±6.20	1.40	0.780
Pro-Caspase 3	107.21 ± 20.89	109.96 ± 19.89	2.75	0.889
Cleaved Caspase 3	40.89 ± 12.79	43.35 ± 6.22	2.46	0.799
Catalase	93.40 ± 24.75	97.04 ± 25.26	3.63	0.880
clAP-1	39.80 ± 20.28	45.09 ± 20.87	5.28	0.792
clAP-2	16.72 ± 4.71	18.86 ± 5.27	2.15	0.630
Claspin	40.49 ± 6.13	43.37 ± 6.39	2.89	0.618
Clusterin	22.70 ± 6.30	27.93 ± 7.19	5.23	0.437
Cytochrome c	68.46 ± 8.61	76.39 ± 8.48	7.93	0.336
TRAIL R1/DR4	40.74 ± 6.48	46.36 ± 3.04	5.62	0.241
TRAIL R2/DR5	66.06 ± 16.01	77.34 ± 6.60	11.28	0.363
FADD	64.20 ± 13.56	71.59 ± 4.95	7.39	0.467
Fas/TNFSF6	66.16 ± 23.27	68.26 ± 13.45	2.10	0.904
HIF-1a	29.40 ± 16.62	41.97 ± 15.61	12.57	0.436
HO-1/HMOX1/HSP32	27.06 ± 14.68	35.64 ± 12.58	8.58	0.526
HO-2/HMOX2	38.85 ± 14.23	49.90 ± 18.08	11.04	0.497
HSP27	33.21 ± 7.16	38.64 ± 9.88	5.43	0.528
HSP60	65.84 ± 11.85	70.83 ± 7.05	4.99	0.545
HSP70	66.75 ± 13.76	64.40 ± 5.59	−2.35	0.801
HTRA2/Omi	63.09 ± 3.83	72.57 ± 4.18	9.48	0.038*
Livin	14.08 ± 2.52	18.83 ± 5.12	4.75	0.223
PON2	28.81 ± 5.92	31.30 ± 5.53	2.49	0.651
p21/CIP1/CDNK1A	27.93 ± 9.01	38.74 ± 9.16	10.81	0.259
p27/Kip1	16.75 ± 6.04	21.34 ± 2.89	4.59	0.345
Phospho-p53 (S15)	82.25 ± 19.34	88.18 ± 11.03	5.93	0.696
Phospho-p53 (S46)	64.83 ± 18.94	71.42 ± 20.27	6.59	0.729
Phospho-p53 (S392)	64.02 ± 28.92	75.33 ± 22.81	11.31	0.659
Phospho-Rad17 (S635)	11.71 ± 2.21	18.72 ± 5.65	7.01	0.129
SMAC/Diablo	56.47 ± 11.12	59.84 ± 9.98	3.36	0.742
Survivin	57.52 ± 23.63	61.96 ± 19.33	4.44	0.831
TNF RI/TNFRSF1A	18.18 ± 4.27	22.05 ± 1.64	3.87	0.210
XIAP	49.96 ± 5.39	56.28 ± 3.45	6.32	0.149

All values are in percent of the calibrator (positive control). The change is given in percent points of the calibrator. The data were acquired in three independent experiments. *: P < 0.05.