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. Author manuscript; available in PMC: 2013 Jul 5.
Published in final edited form as: Virology. 2012 Apr 24;428(2):128–135. doi: 10.1016/j.virol.2012.03.006

Figure 5. The LigIVα2 helix is not sufficient for targeted degradation by adenovirus.

Figure 5

Human LigIV 600–911 was mutated at residue 714 to the mouse amino acid (L714S), and full-length mouse LigIV was mutated at residue 714 to the human amino acid (S714L). 293 cells were transfected with wild type (WT) human (600–911) or mouse (1–911) LigIV and the 714 plasmids. Four hours after transfection, cells were either mock infected (−) or infected with adenovirus (+) for 2hrs. Cells were harvested 20hrs post infection, lysed, and 20μg soluble protein was run on a polyacrylamide gel that was immunoblotted with anti-myc, anti-p53, and anti-actin.