Table 2.
Regions of AAV-2 contacted by MAb A20. Residues were identified that fell within 4 Å of the fitted Fab′ homology model. Most of the footprint is validated by comparison to two independent assessments (Table S1). Parentheses denote residues on the periphery that were not implicated by all three approaches.
| AAV-2 Residues | Subunit | Variable Region | |
|---|---|---|---|
|
|
|||
| Plateau | 261SSQS264 | B | I |
|
| |||
| Ser384, Gln385 | B | III | |
|
| |||
| Val708 | A | IX | |
| Asn717 | A | ||
|
|
|||
| Canyon wall | Lys258 | B | |
|
|
|||
| Canyon | Asn253, Asn254 | B | |
|
| |||
| 658(S)T(T)660 | I | ||
|
|
|||
| Spike | (Glu548, Lys556) | A | VII |