Table 1.

Data Collection and Refinement Statistics

	hSUN2	hSUN2-L546M	hSUN2:KASH1	hSUN2:KASH2
Data collection
Data set	Native	Selenomethionine	Native	Native
Space group	R32	R32	R32	R32
a, b, c (Å)	79.6, 79.6, 199.1	79.7, 79.7, 200.2	79.6, 79.6, 256.4	79.3, 79.3, 260.0
Wavelength (Å)	0.9795	0.9792	0.9792	0.9792
Resolution range (Å)	56.7-2.2 (2.3-2.2)	56.8-2.4 (2.5-2.4)	85.5-2.3 (2.4-2.3)	43.3-2.7 (2.8-2.7)
Total reflections	72,988	56,714	49,146	52,267
Unique reflections	12,305	9,510	13,944	8,912
Completeness (%)	99.8 (99.9)	99.6 (99.8)	99.6 (97.7)	99.9 (99.8)
Redundancy	5.9 (6.1)	3.1 (3.1)	3.5 (3.4)	5.9 (6.1)
Rmerge(%)	4.8 (57.0)	4.6 (65.0)	7.6 (70.7)	5.5 (69.5)
Rr.i.m.(%)	5.3 (62.3)	6.6 (78.8)	9.0 (83.3)	6.1 (76.1)
Rp.i.m.(%)	2.1 (24.8)	2.7 (31.8)	4.7 (43.4)	2.5 (30.7)
I /σ	20.0 (3.5)	22.2 (2.5)	24.9 (1.5)	21.0 (2.7)
Wilson B factor (Å2)	53.0	57.9	45.4	66.5
Refinement
Resolution range (Å)	56.7-2.2		45.0-2.3	36.1-2.7
Rwork (%)	18.9		19.6	18.4
Rfree (%)	23.3		21.1	23.9
Number of Reflections
Total	12,304		13,930	8,909
Rfree reflections	1,250		1,393	891
Number of Atoms
Protein	1,585		1,762	1,751
Water	101		129	41
n-Decyl-β-maltoside			25
B Factors (Å2)
SUN2	45.8		44.8	65.0
KASH			59.8	93.2
Water	48.2		50.4	61.6
n-Decyl-β-maltoside			71.7
R.m.s. Deviations
Bond length (Å)	0.009		0.007	0.009
Bond angles (°)	1.170		1.023	1.141
Ramachandran Plot
Favored (%)	94.5		94.5	93.1
Allowed (%)	5.5		5.5	6.4
Outliers (%)	0		0	0.5

The highest resolution shell is in parenthesis. R_merge is the merging R factor. R_r.i.m. is the redundancy independent merging R factor.

R_p.i.m. is the precision-indicating merging R factor. For definitions, see Weiss (2001).