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Inhibition of WT-PF4 with the nontetramerizing mutant PF4K50E. Inhibition of PF4 tetramerization (A), ULC formation (B), and FcγRIIA activation (C). PF4 concentrations were maintained constant by diluting WT PF4 with PF4K50E. Data are the mean ± SEM of at least 3 independent experiments performed in triplicate.
