Fig. 2.

Preincubation enhances the potentiating effects of enflurane on taurine-activated GlyRs. A, sample tracings showing enflurane potentiation of taurine responses with and without preincubation. The left tracing shows coapplication of 100 mM taurine and 1.2 mM enflurane, with applications of 100 mM taurine alone (before and after). The right tracing shows the effect of preincubation with 1.2 mM enflurane, immediately followed by coapplication of 100 mM taurine and 1.2 mM enflurane. Applications of 100 mM taurine alone preceded and followed the enflurane response. Horizontal bars over tracings indicate the time of exposure to taurine or enflurane. B, summary of the effects of 100 mM taurine and 1.2 mM enflurane with and without preincubation. The y-axis represents the percentage increase in current observed in the presence of enflurane compared with that produced by taurine alone. Data are shown as mean ± S.E.M. of five oocytes. ***, P < 0.001.