Fig. 5.

Inhalants affect currents elicited by maximally effective concentrations of taurine but not glycine. A, sample tracing demonstrating the effects of maximally effective concentrations of glycine and taurine applied with or without 0.39 mM TCY. The tracing shows 30-s preincubations with TCY followed immediately by 15-s applications of either 10 mM glycine or 100 mM taurine plus TCY. Each of these applications was preceded and followed by applications of 10 mM glycine or 100 mM taurine, respectively. Horizontal bars over tracings indicate time of exposure to glycine, taurine, or TCY. B, summary of the effects of various inhalants on 15-s applications of maximally effective concentrations of glycine or taurine. The y-axis represents the percentage change in current observed in the presence of inhalant compared with that produced by glycine or taurine applied alone. Data are shown as mean ± S.E.M. of five to seven oocytes for each inhalant. C, sample tracings showing the effects of 0.56 mM TCE coapplied with saturating concentrations of glycine or taurine, after the neurotransmitters had already been applied for 10 min. In the top tracing, 0.56 mM TCE was coapplied with 10 mM glycine after 10-min exposure to a saturating glycine concentration. The bottom tracing shows the same for a sustained application of 100 mM taurine instead of glycine. D, summary graph of the effects of various inhalants during long applications of saturating concentrations of glycine and taurine. The y-axis represents the percentage change in current observed in the presence of inhalant compared with the glycine or taurine current seen immediately before inhalant coapplication. Data are shown as mean ± S.E.M. of four to eight oocytes.*, P < 0.05, **, P < 0.01, ***, P < 0.001 comparing glycine with taurine.