Figure 6.

Co-expression of lymphoid and peripheral homing receptors on CXCR3⁺ Tregs. (A) PBMCs were stained with antibodies to CD4, CD25, FOXP3 and either CXCR3, CD62L, CCR4, CCR5 or CCR7. Gating was performed either on the CD4⁺CD25^hiFOXP3⁺ Treg subset (grey box plots) or on the CD25^loFOXP3⁻ T effector subset (white box plots). Illustrated is the median, 25th and 75th percentile and range of expression of each homing receptor in n≥6 donors, as indicated. (B) Purified populations of CD4⁺ T cells were stained with antibodies to CD25, FOXP3 and CXCR3, and either the lymphoid homing receptors CD62L and CCR7 or the peripheral homing receptors CCR4 or CCR5. After gating on CD4⁺CD25^hiFOXP3⁺ cells, co-expression of CXCR3 with CD62L, CCR7, CCR4 and CCR5 is illustrated (representative of n = 4 with similar results).