Table 1.
Plasma levels of cytokines/heat shock proteins after polytrauma.
| Marker (pg/mL) |
Baseline | Shock (t=1-60 min) |
Resuscitation (t=61-300 min) |
|---|---|---|---|
|
| |||
| IL-1β | |||
| Mean ± SD | 0 | 0 | 0 |
| Median (Min – Max) | 0 (0) | 0 (0) | 0 (0) |
|
| |||
| IL-4 | |||
| Mean ± SD | 0.1 ± 0.13 | 0.08 ± 0.05 | 0.05 ± 0.09 |
| Median (Min – Max) | 0.05 (0 – 0.31) | 0.11 (0.02 – 0.13) | 0 (0 – 0.24) |
|
| |||
| IL-5 | |||
| Mean ± SD | 39.3 ± 12.5 | 49.2 ± 9.7 | 41.6 ± 13.3 |
| Median (Min – Max) | 37.8 (24 – 58) | 48.7 (35 – 60) | 50.1 (23 – 52) |
|
| |||
| IL-6 | |||
| Mean ± SD | 134 ± 7 | 141 ± 8 | 188 ± 15a |
| Median (Min – Max) | 131 (128 – 145) | 142 (131 – 152) | 184 (170 – 206) |
|
| |||
| IL-8 | |||
| Mean ± SD | 0 | 12 ± 29 | 2.2 ± 4.2 |
| Median (Min – Max) | 0 (0) | 0 (0 – 71) | 0 (0 – 10.5) |
|
| |||
| IL-10 | |||
| Mean ± SD | 15.3 ± 14.2 | 80.9 ± 63.6 | 90.7 ± 56.4a |
| Median (Min – Max) | 8.9 (0 – 31) | 76.3 (15 – 174) | 92.9 (39 – 175) |
|
| |||
| IL-12/IL-23 p40 | |||
| Mean ± SD | 739 ± 268 | 791 ± 257 | 900 ± 294 |
| Median (Min – Max) | 654 (582 – 1213) | 681 (628 – 1245) | 857 (621 – 1389) |
|
| |||
| IL-13 | |||
| Mean ± SD | 6.5 ± 3.4 | 9.6 ± 3.1 | 7.2 ± 2.3 |
| Median (Min – Max) | 6.2 (2.5 – 11.1) | 10 (5.4 – 13.6) | 66.5 (5.7 – 11.2) |
|
| |||
| IL-17 | |||
| Mean ± SD | 0 | 0.5 ± 1.1 | 0.4 ± 0.9 |
| Median (Min – Max) | 0 (0) | 0 (0 – 2.5) | 0 (0 – 1.9) |
|
| |||
| IL-18 | |||
| Mean ± SD | 0 | 0 | 0 |
| Median (Min – Max) | 0 (0) | 0 (0) | 0 (0) |
|
| |||
| IFNγ | |||
| Mean ± SD | 0 | 0 | 0 |
| Median (Min – Max) | 0 (0) | 0 (0) | 0 (0) |
|
| |||
| TNFα | |||
| Mean ± SD | 133 ± 69 | 261 ± 186 | 249 ± 60a |
| Median (Min – Max) | 122 (44 – 265) | 199 (54 – 574) | 249 (153 – 327) |
|
| |||
| TGFβ | |||
| Mean ± SD | 926 ± 407 | 1006 ± 508 | 1084 ± 861 |
| Median (Min – Max) | 1019 (173 – 1289) | 1033 (395 – 1731) | 866 (422 – 2770) |
|
| |||
| HSP40 | |||
| Mean ± SD | 0.41 ± 0.5 | 0.04 ± 0.06 | 0 |
| Median (Min – Max) | 0.25 (0 – 1.2) | 0 (0 – 0.16) | 0 (0) |
|
| |||
| HSP70 | |||
| Mean ± SD | 144 ± 48 | 155 ± 29 | 193 ± 43b |
| Median (Min – Max) | 169 (79 – 189) | 168 (122 – 184) | 216 (145 – 234) |
|
| |||
| HSP90 | |||
| Mean ± SD | 3.4 ± 1.0 | 3.4 ± 0.6 | 3.8 ± 0.9 |
| Median (Min – Max) | 3.4 (1.9 – 4.8) | 3.5 (2.6 – 4.2) | 3.3 (3.3 – 5.4) |
Average plasma concentrations at baseline, during shock and resuscitation (n = 5 per time period).
a
p<0.05 vs. baseline;
b
p<0.05 vs. shock. The average plasma concentration for each animal was calculated as the mean of two, eight and three plasma levels that were determined at baseline, during shock and resuscitation, respectively.