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. Author manuscript; available in PMC: 2012 Jun 26.
Published in final edited form as: Dev Cell. 2010 May 18;18(5):725–736. doi: 10.1016/j.devcel.2010.02.017

Figure 2. FAK, Src, and Rac activity are necessary for LN-dependent neuritogenesis.

Figure 2

(A) E14.5 e/v cortical neurons cultured 48 hours expressing FP4Mito (blue), and either the FAK inhibitor (FRNK, green), constitutively active Src (Y527F, green) or treated with the indicated drugs, plated on PDL or LN. Neurons not expressing FRNK or SrcY527F were stained for βIII tubulin (green) and phalloidin (Red). (B) Quantification of morphologies, as in Fig 1. Targeted molecules include PI3K, (50 μM LY294002, LY), PKA (20 μM H-89), PKC (1 μM GF109230X, GF), Src (50 nM PP2), PLD (0.3% 1-butanol, 1-b). n≥ 3 independent experiments/treatment, ≥25 neurons/experiment. Scale bar is 10 μm (C) E14.5 neurons cultured 48 hours, expressing AP4Mito (CON) or FP4Mito (e/v) (blue) plated on PDL or LN. Red is phalloidin and green indicates either anti-myc (for Rac mutants), GFP (for Cdc42 mutants) or βIII tubulin, in neurons not expressing a mutant. (D) Quantification of morphologies +/- SEM. n ≥ 3 independent experiments/treatment, ≥25 neurons/experiment. Scale bar is 10 μm.