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. Author manuscript; available in PMC: 2012 Jun 26.
Published in final edited form as: Dev Cell. 2010 May 18;18(5):725–736. doi: 10.1016/j.devcel.2010.02.017

Figure 4. VAMP2 and VAMP7 are required for Ena/VASP-dependent and LN-dependent, Ena/VASP-independent neuritogenesis, respectively.

Figure 4

(A) neurons expressing AP4Mito (CON) or FP4Mito (e/v) (blue) plated on PDL or LN and cultured 48 hours. (B) Quantification of neuronal morphologies +/- SEM. n ≥ 3 independent experiments/treatment, n ≥ 25 neurons per experiment. 50 nM tetanus neurotoxin (TeNT) and expression of the NH2 domain of VAMP7 (NH2-V7) ere used to block VAMP2 and VAMP7-mediated exocytosis, respectively. Overexpression of Exo70 or VAMP7 but not VAMP2 nor VAMP3 rescued neuritogenesis in e/v neurons on PDL. VAMP expression is green. Phalloidin is red; neurons not expressing VAMP were stained for βIII tubulin (green). Scale bar is 10 μm. (C) Summary of molecules implicated in Ena/VASP-dependent or LN-dependent neuritogenesis.