Figure 7. .

Comparison of morphometry by histology and SD-OCT in ouabain-damaged retinas. Morphometric comparisons of whole retina (A, B), inner retina (C, D), and outer retina (E, F) were plotted. Scatterplots of whole retina thickness (A), inner retina (C), and outer retina (E) measurements by histomorphometry (x-axis) against SD-OCT (y-axis) of the same fish. Bland–Altman plots depict the agreement between histomorphometry against SD-OCT of the whole retinal thickness (B), inner retina (D), and outer retina (F). The middle red line on the Bland–Altman plots represents the mean difference and is bound by the 95% CI (flanking red lines). (A) Best fit line of measurements over the whole retina showed a correlation (R² = 0.641). (B) The mean difference of histology compared with SD-OCT was −9 ± 65 μm (95% CI) over the average thickness of 189 μm. (C) Best fit line of measurements over the inner retinal thickness showed a correlation (R² = 0.784). (D) The mean difference of histology compared with SD-OCT was 6 ± 31 μm (95% CI) over the average inner retinal thickness of 78 μm. (E) Best fit line of measurements over the outer retinal thickness showed a correlation (R² = 0.243). (F) The mean difference of histology compared with SD-OCT was −7 ± 23 μm (95% CI) over the average outer retinal thickness of 110 μm.