Figure 3.

Effect of acute urea loading on urinary concentrating activity and renal handling of urea in WT, UT-B KO, and UT-A2/B KO mice. Three-hundred micromolar urea were injected intraperitoneally just after the first 2 h urine collection (time 0), and urine was then collected for four more 2 h periods. (A) Urine osmolality (U_osm). (B) Urine output. (C) Urine urea concentration (U_urea). (D) Plasma urea concentration P_urea. Values are expressed as means ± SE, n = 6/group. *p < 0.01 compared with WT mice, ^#p < 0.01 compared with UT-B KO mice. Reproduced from Lei et al. (2011).