Table 2.

Amino acid and nucleotide sequences of high-frequency clonotypes derived from replicate mice with GVHD-associated autoimmunity

Experiment	Vβ family	Jβ segment	Clonotype name	Nucleotide sequence	Translation
1	1	1.4	sPGQs.231B1B14L12	AGCCCCGGACAATCC	CASSPGQSNERLFFG
	4	1.1	sLGTDn.3431B4B11L12	AGCCTTGGGACAGATAAC	CASSLGTDNTEVFFG
	6	2.12	sIWGn.214B6B21L12	AGTATCTGGGGGAAC	CASSIWGNYAEQFFG
	6	2.1	iGAGEy.4342B6B21L13	ATAGGGGCAGGGGAGGAGTAT	CASSIGAGEYAEQFFG
	8.1	2.5	dAREPq.3612B8.1B25L10	GATGCAAGGGAACCCCAG	CASSDAREPQYFG
	8.1	2.2	sGRn.45B8.1B22L11	AGTGGGAGAAAC	CASSGRNTGQLYFG
	8.1	2.6	sWDRVy.1262B8.1B26L11	AGTTGGGACAGGGTCTAT	CASSGRNTGQLYFG
	8.1	2.2	dALGGt.1644B8.1B22L13	GATGCTCTGGGGGGGACC	CASSDALGGTGQRYFG
	15	1.3	gASDSs.2225B15B13L11	GGTGCCTCCGACAGTTCT	CGASDSSGNTLYFG
2	5.2	2.3	sLEGDt.6242B5.2B11L11	TCTCTGGAGGGGGACACA	CASSLEGDTEVFFG
	6	1.3	sKTDg.211B6B13L10	AGCAAGACTGATGGA	CASKTDGNTLYFG
	6	2.1	iGTGGy.4144B6B21L13	ATAGGGACTGGGGGGTAT	CASSIGTGGYAEQFFG
	8.1	2.4	sDGWGq.2214B8.1B24L12	GACGGCTGGGGGCAA	CASSDGWGQNTLYFG
	12	2.6	sDWGGy.2144B12B26L11	AGTGACTGGGGGGGGTAT	CASSDWGGYEQYFG
	12	2.1	lTGQy.232B12B21L12	TTAACCGGACAGTAT	CASSOTGQYAEQFFG
3	1	1.1	qEGGn.141B1B11L12	CAAGAAGGGGGTAAC	CASSQEGGNTEVFFG
	8.1	2.6	dAGNy.441B8.1B26L11	GATGCGGGGAATTAT	CASSDAGNYEQYFG
	8.1	2.5	sAGGAd.1442B8.1B25L11	AGTGCTGGGGGGGCCGAC	CASSAGGADTQYFG
	8.1	2.2	sPGQGDt.43242B8.1B22L13	AGTCCGGGACAGGGGGACACC	CASSPGQGDTGQLYFG
	10	1.3	sLTDSGDt.231131B10B13L12	AGCTTGACAGATTCTGGAGATAC	CASSLTDSGDTLYFG
	10	2.1	sSTWGn.4212B10B21L13	AGCTCGACCTGGGGCAAC	CASSSTWGNYAEQFFG
	15	1.5	aATGDq.4342B15B15L10	GCTGCGACAGGGGACCAG	CGAATGDQAPLFG
	15	2.6	rESs.26B15B26L10	AGGGAGAGCTCC	CGARESSYEQYFG
	15	2.4	rSs.5B15B24L10	AGGAGTAGT	CGARSSQNTLYFG
	15	1.4	rDTGe.112B15B14L10	AGGGATACTGGCGAA	CGARDTGERLFFG
	15	2.6	aSLGGPy.66432B15B26L11	GCTAGCCTGGGGGGCCCCTAT	CGASLGGPYEQYFG

Amino acid and nucleotide sequences of high-frequency clonotypes that were obtained from the colons of mice with pathologic evidence of colitis. Data are derived from 3 independent experiments in which mice received either unseparated spleen cells adjusted to yield a fixed αβ T-cell dose (experiments 1 and 2 in Table 1) or purified CD4⁺ T cells (experiment 3 in Table 1). Nucleotide sequences in bold correspond to the bold amino acid sequence. Clonotypes were defined using the nomenclature described in Yassai et al.²⁷