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Localized surface plasmon resonance spectra for each step of the detection of 500 pM human epididymis secretory protein 4. (A) Bare silver nanochip, λmax = 592.58 nm, (B) 1 mM 11-mercaptoundecanoic acid, λmax = 619.85 nm, (C) functionalized biosensor with 10 μg/mL antibody, λmax = 630.97 nm, and (D) 500 pM HE4, λmax = 645.45 nm.
Note: All spectra were collected at room temperature in air.
Abbreviation: HE4, human epididymis secretory protein 4.
