Figure 3.

Archaeosome-entrapped SAgs induce Th1 biased response in immunized mice. By determining cytokine response in splenocyte culture supernatant of various immunized groups, Th1/Th2 response was ascertained at different time points, prior and post-challenge with Listeria monocytogenes infection; (A) IFN-γ, (B) IL-12, (C) IL-4. (D) Ratio of IgG2a to IgG1 was analyzed in sera of various immunized groups except controls (PBS and sham archaeosomes) to further confirm TH1/Th2 polarization upon immunization with archaeosome-entrapped SAgs.

Notes: The data represent the mean of three determinants ± SD and are representative of three different experiments (ie, the experiment was done in triplicate) with similar observations. Statistically groups were compared with each other using ANOVA with the Holm–Sidak test (all pairwise multiple comparison procedures) with P < 0.05, P < 0.01, P < 0.001, post-booster, and post-challenge.

Abbreviations: PBS, phosphate buffer saline; SD standard deviation; ANOVA, analysis of variance; SAgs, secretory protein antigens.