TABLE 2.
FFA content in control and PLA2-treated LDL samples after fatty acid supplementation
| FFA Mix |
|||
|---|---|---|---|
| Sample | 0 mM | 0.04 mM | 0.2 mM |
| Control | −0.015 ± 0.0003 | 0.003 ± 0.000 | 0.010 ± 0.0049a |
| PLA2 | 0.141 ± 0.0387 | 0.169 ± 0.0599 | 0.224 ± 0.0044a |
LDL samples were incubated with the indicated concentrations of fatty acid mixture [oleic, linoleic, and arachidonic acid (20:40:10)] for 4 h, and the unbound material was removed by ultracentrifugation in the presence of 1% BSA. Data show higher enrichment of PLA2-LDL samples with unesterified fatty acids. Values are expressed as μmol/mg LDL protein.
P < 0.05 compared with 0 mM FFA concentration.