Figure 2.

Biocompatibility of c(RGDfC) polyionic complex micelles with neurons. Neurons stained with Hoechst 33258 for nucleus and TUNEL for apoptosis after coculture with vehicle (A) or c(RGDfC) polyionic complex micelles (B) for 24 hours (bar 40 μm). Seven-day primary cultured neurons in each well of 6-well dishes were treated with c(RGDfC) polyionic complex micelles (150 μmol/mL) for 24 hours, stained with Hoechst 33258 and TUNEL and imaged under fluorescent microscope. (C) Cell viability of neurons following dose-dependent treatments determined by MTT assay. Seven-day primary neurons were given as 0, 50, 150, 300 and μmol/mL c(RGDfC) polyionic complex micelles, c(RGDfC), or polyionic complexes, respectively, for 4 hours. The cells were subjected to MTT assay following treatment. No significant reduction in cell viability was observed in the neurons with the c(RGDfC) polyionic complex, c(RGDfC), or polyionic complex micelles. (D) Viability of the neurons following time-dependent treatments determined by MTT assay. Neurons were cocultured with 150 μmol/mL c(RGDfC) polyionic complex, c(RGDfC), or polyionic complex micelles for a series of times, ie, 0, 2, 24, and 72 hours. The cells were subjected to MTT assay following treatment. No significant reduction of cell viability was observed in neurons treated with the c(RGDfC) polyionic complex, c(RGDfC), or polyionic complex micelles.

Note: All cellular experiments were repeated at least three times as confirmation.

Abbreviation: c(RGDfC), Cyclo(-Arg-Gly-Asp-D-Phe-Cys).