Figure 3.

Inhibition of U87 glioma-derived cells after treatment with c(RGDfC) polyionic complex micelles. Apoptosis of U87 cells post treatment with c(RGDfC) polyionic complex micelles. Three-day U87 cells were treated with (A) phosphate-buffered saline, (B) 150 μmol/mL of polyionic complexes, (C) 150 μmol/mL of c(RGDfC), and (D) 150 μmol/mL of c(RGDfC) polyionic complex micelles for 4 hours and imaged under microscopy (bar 125 μm in each graph), and (E) viability of U87 cells post treatment, determined by MTT assay. To see whether the compound kills glioma cells, 3-day U87 cells cultured in 96-well plates were divided into 12 groups (n = 5), receiving 0, 50, 150, 300 μmol/mL c(RGDfC) polyionic complex micelles, c(RGDfC), or polyionic complexes for 4 hours.

Notes: The cells were subjected to MTT assay post treatment. Significant reduction of cell viability was observed in the group treated with c(RGDfC) polyionic complexes (^#P < 0.05) and the group treated with c(RGDfC) (*P < 0.05), while no significant reduction of cell viability was seen in the group treated with polyionic complex micelles. All cellular experiments were repeated at least three times as confirmation.

Abbreviation: c(RGDfC), Cyclo(-Arg-Gly-Asp-D-Phe-Cys).