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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Mol Microbiol. 2012 May 24;85(1):21–38. doi: 10.1111/j.1365-2958.2012.08081.x

Figure 1.

Figure 1

Widefield imaging of ribosomes (S2-YFP labels) and chromosomal DNA (DRAQ5 stain) for K-12 cells grown in EZRDM at 30°C. (A) Ribosome distribution (green) and DNA distribution (red) for three typical cells. The composite image shows the anti-correlation between the two distributions. (B) Axial intensity distributions in the ribosome and DNA channels for a short, medium, and long cell. Intensity is summed along y (short axis coordinate) at each x (long axis coordinate). The two channels are strongly anti-correlated. (Inset: Phase contrast image of a cell showing x (along the long axis) and y (along short axis) coordinates.) (C) DNA axial intensity distribution for a short, medium, and long cell plotted on same axes to show progressive segregation as the cell elongates. (D) The distance from the cell center of the local maxima in ribosome distribution (green dots) and DNA distribution (red dots) plotted for 286 cells of different cell length. The black dashed lines guide the eye. Scale bar = 1 μm.

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