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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Mol Microbiol. 2012 May 24;85(1):21–38. doi: 10.1111/j.1365-2958.2012.08081.x

Figure 2.

Figure 2

Widefield imaging of RNA polymerase (β′-yGFP labels) and chromosomal DNA (DRAQ5 stain) for K-12 cells grown in EZRDM at 30°C. (A) Phase contrast image, RNAP image, and DNA image are shown for two different cells. The composite image shows RNAP co-localizes with DNA. Scale bar is 1 μm. (B) Axial intensity profile for RNAP-yGFP and DRAQ5-labeled DNA compared from a single cell. (C) RNAP images from three cells of length (i) 2.6 μm, (ii) 3.9 μm, and (iii) 5.1 μm. Scale bar is 1 μm. Progressive segregation of RNAP distribution is similar to DNA segregation shown in Fig. 1.

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