Figure 3.

Superresolution images of ribosomes (S2-YFP) within K-12 cells grown in EZRDM at 30°C. Each localization is plotted as a point at the calculated centroid position. (A) Nine representative cells. (B) Image of two single molecules within a cell prior to image filtering. Cell outline based on phase contrast image. (C) Expanded view of superresolution image of ribosomes in the same cell. A model spherocylinder is shown as a guide to the endcap positions. (D) Relative number of ribosomes at each axial position, with data along y at each x summed into 100 nm bins. The grey background shows the theoretical profile for a uniform distribution filling the model spherocylinder, taking account of measurement uncertainty and binning. Sectioning by the 1.49 NA objective does not affect the axial distribution significantly, as shown in Supporting Information (Fig. S3).