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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Mol Microbiol. 2012 May 24;85(1):21–38. doi: 10.1111/j.1365-2958.2012.08081.x

Figure 4.

Figure 4

Superresolution images of RNA polymerase (β′-yGFP) within K-12 cells grown in EZRDM at 30°C. (A) Four representative cells. (B) Expanded view of a medium-length cell (tip-to-tip length = 3.9 μm). (C) Expanded view of a long cell (tip-to-tip length = 5.2 μm). (D) Relative number of RNAP copies at each axial position x (100 nm bins). Solid line is the result of a Savitzky-Golay smoothing filter. The grey background shows the theoretical profile for a uniform distribution filling the model spherocylinder, as in Fig. 3. Note that RNAP avoids the endcaps.

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