Figure 6.

Light microscopy images (original magnification 100×) of hematoxylin and eosin-stained liver and spleen tissues from toxicity analysis in female C57Bl/6 mice.

Notes: No histopathological changes were observed in liver (A and B, day 13; E and F, day 28) and spleen (C and D, day 13; G and H, day 28) following administration of free cisplatin at a dose of 4 mg/kg, cisplatin-loaded cl-micelles at a cisplatin equivalent dose of 4 mg/kg, or empty micelles at 2.5 mg polymer/kg body weight. Treatment was administered by bolus intravenous tail vein injection (n = 5), four treatments in total, with each treatment at a 4-day interval. The animals were sacrificed using CO₂ euthanasia on day 13 and day 28 for toxicity analysis. Hematoxylin and eosin staining was performed as described in materials and methods.