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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Mol Cell Neurosci. 2012 May 8;50(2):160–168. doi: 10.1016/j.mcn.2012.05.002

Fig. 4.

Fig. 4

Co-expression of Brn3b and Isl1 during or shortly after the terminal cell cycle. (A–C) Sections from E11.5, E13.5, and E16.0 embryos co-stained for Brn3b, Isl1 and EdU following a 30 min chase. At E11.5 (A) and E13.5 (B), multiple Brn3b+ Isl1+ EdU+ cells are observed (arrows), indicating that RGC markers can initiate expression prior to cell cycle exit, in late S or G2 phase. In contrast, at E16.0 (C), all Brn3b+ Isl1+ cells are EdU− (examples marked by arrowheads). The insets show orthogonal Z-stack views of magnified Brn3b+ Isl1+ EdU+ cells. Scale bar, 50 μm.