Table 4.
Networks and gene clusters identified by Ingenuity Pathway Analysis of significant differences in higher or lower gene expression in the VTA of the HEC compared to the LEC lines
| Network/gene cluster | Higher Expressiona | Lower Expressionb |
|---|---|---|
| Network 1: neurological disorder; neuronal death; interactions clustered around App | 21 genes: Ank3, Appb1*, Sorl1*, Dnm1l*, Sparcl1*, Aplp2*, Taz*, Pgam1, Bcl2l2*, Apba2*, App*, Erp44*, Kif1a, Hmox2*, Ccdc92*, Git1, Nbl1*, Cfb*, Sncb*, Rtn3*, Lingo1* | 13 genes: Nfia, Rgnef, Gusb, Rp2, Arl4d, Vps33a*, Gas7, Hyou1*, Clic1*, C1qa*, Nptx1*, Ngb*, Fam160a2 |
| Network 2: migration of cells; proliferation of cells; interactions clustered around Egfr | 19 genes: Rab6b, Bmpr1a, Slc9a3r2, Smad7*, Gref1*, Adora1, Cdh2, Rhot2, Bcan*, Sh2b1*, Sorbs1, Rnf41*, Adam9*, Ptprs*, Grb14*, Capza2*, Tom1l1*, Surf4, Cops7a | 15 genes: Chn1, Adora2b, Sash3, Acvr1, Fancl, Col3a1*, Epb41l2, Col6a1*, Trak2*, Axl*, Egfr*, Cd2ap*, Itga9, F11r, Degs1* |
| Network 3: proliferation of cells; transcription of DNA; interactions clustered around Ccnd1 | 25 genes: Anapc2, Nedd9, Rfxap, Id2*, Hla-dqa1, Dnajb1, Cirbp*, Sox2*, Cdk9*, Suds3, Appl2*, Hexim1*, Zeb2*, Ccnk, Ccnd1*, Runx1t1, Krt10*, Supt5h, Tubb3, Ctnnbip1, Mcf2l*, Heafx, Luc7l3, Mdc1, Thoc4 | 10 genes: Anapc10, Hinfp, Mdfic, Hdac1, Csf1r*, Ccndbp1*, Rilpl2, Srpk2*, Cbfb, Phip |
| Network 4: growth & outgrowth of neurites; migration of neurons; interactions clustered around Itga2b | 23 genes: Cir1, Nt5e, Calcb, Vcan, Ncor2, Zbtb7a, Gpx1, Sec23b, Gmnn, Sparc, Hif1a*, Tesk1, Spred1, Pdia4, Itga2b*, Mta3, Mbd3*, Myl6b*, Pc*, L1cam*, Cntn2, Aup1*, Bat5 | 12 genes: Mfng, Jag1, Nov, Mgp, Fn1*, Adamts1, Blnk, Rragd, Vwf*, Crkl, Kidins220, Wdr77 |
| Network 5: interactions clustered around Rxra | 17 genes: Map4k2, Sox4, Nr2c1, Rxrb, Dgkz, Cpt1a*, Rxra*, Znhit3*, Ppargc1a*, Dlat, Tnrc6b, Hmgcs2*, Pcsk2*, Pkn1Ubqln4*, Ncoa4*, Zfpm2*, | 16 genes: Rhoc, Pnlip, Rock2, Leprot, Aprx, Lepr, Slco1a2*, Rbp1*, Cript, Acat1, Ubr7, Tfam, Jazf1*, Slit3, Pdk1, Pmch |
| Network 6: interactions clustered around Vcl | 19 genes: Cog5, Ppp1r12c, Cdc42ep1, Golga2, Letm2*, Tmed2, Adam19, Nrg1*, Vcl*, Ddx19a, Pdcd4, Pabpc1*, Psma2*, Vdac1, Csde1, Nudc, Pcbp1, Hla-c, Nfyc | 16 genes: Cog4, Lpcat3, Scfd1*, Dad1, Snrpf*, Bag4*, Psma4, Pik3r1*, Upf3b, Eif4g2, Eif4g1, Psmc4, Lig3, Psmc6, Psmd6, Tapbp |
a
Genes with higher expression in high vs. low ethanol consuming lines.
b
Genes with lower expression in high vs. low ethanol consuming lines.
*
Indicates each gene that is associated with the cluster within that particular network.