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. Author manuscript; available in PMC: 2013 Aug 16.
Published in final edited form as: Neuroscience. 2012 Apr 30;217:56–66. doi: 10.1016/j.neuroscience.2012.04.054

Figure 5. The distal C-terminus of Kv2.1 is required for clustering but not for modulation by NMDARs.

Figure 5

A. (Left) Maximum intensity projections of HEK293 cells expressing either full-length GFP-Kv2.1 or GFP-Kv2.1-ΔC318. (Right) Representative whole cell currents from GFP-Kv2.1 or GFL-Kv2.1-ΔC318 expressing HEK293 cells. Scale bars: 50 ms, 1 nA. B. Average normalized I-V curves for Kv2.1 and Kv2.1-ΔC318 ± calf intestinal phosphatase (CIP), illustrating that Kv2.1-ΔC318 retains functional sensitivity to phosphorylation state. C. Representative whole-cell currents from HEK293 cell expressing GFP-Kv2.1-ΔC318 plus NMDAR at the indicated time points before and after the addition of 10 μM glutamate. Average normalized I-V curves (D) and V1/2 (E) for Kv2.1-ΔC318 before and after activation of NMDAR. #, p < 0.05, compared to Control; *, p < 0.001, compared to –glut.