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. Author manuscript; available in PMC: 2013 Jul 1.

Published in final edited form as: J Am Coll Surg. 2012 May 24;215(1):126–135. doi: 10.1016/j.jamcollsurg.2012.02.021

Correlation of imaging with histology for documentation of tumor resection using either bright light or fluorescence-guided surgical techniques. Brightfield (lower) and fluorescence (upper) images obtained at the time of surgery for either (A) a bright light or (B) fluorescence-guided surgical approach. Histologic cross-sections (top image in each 6A and 6B, H&E) taken through the surgical margins of the unresected tissues. The arrows in Figure 6A show the correlation of the small focus of unresected (fluorescent) tumor cells retained in the post-operative tumor bed and the histologic section through this focus in a mouse resected for tumor under BLS.

Correlation of imaging with histology for documentation of tumor resection using either bright light or fluorescence-guided surgical techniques. Brightfield (lower) and fluorescence (upper) images obtained at the time of surgery for either (A) a bright light or (B) fluorescence-guided surgical approach. Histologic cross-sections (top image in each 6A and 6B, H&E) taken through the surgical margins of the unresected tissues. The arrows in Figure 6A show the correlation of the small focus of unresected (fluorescent) tumor cells retained in the post-operative tumor bed and the histologic section through this focus in a mouse resected for tumor under BLS.