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. Author manuscript; available in PMC: 2013 Jun 22.
Published in final edited form as: Cell. 2012 May 17;149(7):1635–1646. doi: 10.1016/j.cell.2012.05.003

Figure 3. Regulation of m6A Levels in Cells and During Development.

Figure 3

A. Ontogeny of m6A abundance throughout brain development. Total RNA was isolated from mouse brain at embryonic day 18 (E18), postnatal day 0 (P0), postnatal day 14 (P14), and adulthood, then subjected to immunoblot analysis to detect m6A-containing transcripts. Ethidium bromide staining of 28S rRNA bands is shown as a loading control.

B. FTO demethylates a wide range of cellular transcripts. FTO was expressed in HEK293T cells for 48h, and cellular RNA was subjected to immunoblot analysis to detect m6A.

See also Figure S1.