Fig. 1.
A–B. Extraocular muscle (EOM) preparation and fluo-4 loaded myofibers. A. A chicken superior oblique EOM is pinned to the bottom of a 35-mm dish (dorsal surface). The trochlear nerve is indicated and its branching fibers in the proximal and middle segments are clearly visible. The transition from myofiber to tendon is apparent by the lighter color of the tendon. The thickness of the EOM decreases from proximal to distal. The relative size of the field of view (FOV) used for calcium imaging is shown by the dashed square in the distal region. The larger rectangle within the square represents the size of the image in (B). B. Representative image of fluo-4 dye-loaded extraocular myofibers in the distal segment of a superior oblique muscle from a 9-day old chicken.