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. 1998 Mar 3;95(5):2017–2020. doi: 10.1073/pnas.95.5.2017

Figure 2.

Figure 2

The differential hybridization between maize and sorghum can be used as a tool in the identification of single- and low-copy sequences. In maize, the small arm of chromosome 4 contains a large cluster of 20–22 pseudogenes and genes encoding α-zeins, the major group of storage proteins in kernels. Most of the intergenic region in the cluster consists of highly repetitive DNA, many of them retrotransposons (V.L., R. Wing, and J.M., unpublished work). BACs containing sorghum DNA homologous to the 22-kDa cluster were hybridized to 14 cosmid clones from the cluster region isolated from a maize BSSS53 library. (A) Agarose gel electrophoresis of the maize cosmids digested with EcoRI. (B and C) Southern blots from the same gel, hybridized to two different sorghum BACs, M18 and J21, respectively. Fragments cross-hybridizing to sorghum BACs contained single- or low-copy sequences, including zeins and the RFLP marker php20725.