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The use of the smaller sorghum genome for gap closure and contig linking in maize can reduce significantly distances in walking strategies. In sorghum, the distance from the RFLP marker php20725 to the nearest zein-homologue sequences, called kafirins in sorghum, is only 5 kb, at least six times shorter than its counterpart in maize. Most of the difference in distance can be accounted for the presence of large sections of high-copy DNA that includes retrotransposon-like sequences.
