Figure 5. Homing of hUCBSC toward tumors in the mice brains. 4910 GSC (250,000 cells) were injected into the brains of athymic nude mice by intracranial administration. After 2 weeks, hUCBSC (250,000 cells) were injected into either (A) the tail vein or (B) intracranially (contra-lateral) and observed for 3 weeks. From the 2nd week until the end of the 5th week, mice were subjected to in vivo imaging using the IVIS Imaging System (n = 6 for each group). (C) Mice brains were injected with 10 µL of sterile PBS by intracranial administration. After 1 week, labeled hUCBSC (250,000 cells) were injected by through the tail vein and observed for homing by in vivo imaging; n = 3. (D) The brain sections of mice (A, B) were checked for the presence of tumor formation by H&E staining; bar = 500 µm; n = 3. (E) Survival curves were plotted for control and treated mice.