Figure 2. Inhibitory effects of metformin and rosiglitazone on leukemic cell DNA replication. (A) The percentage of cells in S phase as determined by PI flow cytometry measuring DNA content were plotted on the bar chart with treatments labeled on the horizontal axis. The error bars represent 95% confidence intervals. The black bars are data from Reh cells, and white bars, Nalm-6 cells. (B) The percentage of replicating cells in S phase was determined by 2-dimensional flow cytometry measuring BrdU incorporation and DNA content Both metformin and rosiglitazone, at labeled concentrations, decreased the percentage of Reh cells in the S-phase of the cell cycle (red numbers) and the percentage of BrdU-labeled cells with DNA content between 2n and 4n (black numbers) .