Figure 3. Timeless-depleted cells have telomere aberrations. (A, B) Frequency of metaphase telomere phenotypes observed in HeLa (A) or MCF-10A (B) cells expressing control or Timeless shRNA. Metaphase spreads were hybridized with a telomere-PNA probe. n > 100 metaphase spreads; Error bars correspond to standard deviations obtained from three independent experiments. (C) Representative images of a normal metaphase chromosome and telomere abnormalities are shown.