Examples of reporter gene expression (blue) in
various gene trap and enhancer trap lines illustrating cell
type-specific expression, expression in prepatterns, and subcellular
gene trap localization. Seedlings were grown in continuous light for 7
days, stained for GUS activity, cleared in 70% ethanol, and mounted
for differential interference contrast microscopy in 25% glycerol (12,
42). (A) Nuclear staining (arrow) in roots from heterozygous
prolifera/+ plants. (B) Meristem staining
in the shoot. (C) GUS expression at the basal boundaries of
leaf primordia. (D) GUS expression in columella initials in
the root. (E) GUS expression in root cap cells.
(F) GUS expression in immature trichomes. (G) GUS
expression in trichome accessory cells.