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Activities of purified Δ-6 protoilludene synthases Omp6 and Omp7. (a) Kinetic parameters for Omp6 and Omp7 were determined using (E,E)-FPP as the substrate in a coupled spectrophotometric assay. (b) GC-MS analysis of in vitro reactions containing purified Omp6 or Omp7 incubated with (E,E)-FPP. Both enzymes produce greater than 99 % Δ-6 protoilludene. Omp7 also produces a very small amount of pentalenene 1.
