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. 2012 Jun 26;7(6):e39658. doi: 10.1371/journal.pone.0039658

Figure 4. Comparison between the GLO isoforms expressed in yeast and those extracted from rice leaves.

Figure 4

GLO isozymes were separated in 6% uniform CN-PAGE at a running pH of 10.2. Y-GLO1, Y-GLO3, Y-GLO4, represent the enzyme extracted from yeast cells expressing pYES3-GLO1, pYES3-GLO3, pYES3-GLO4, respectively. Y-GLO3+4, Y-GLO3+1, Y-GLO1+4, represent the enzyme extracted from yeast cells co-expressing pYES3-GLO3 and pYES2-GLO4, pYES3-GLO3 and pYES2-GLO1, pYES3-GLO1 and pYES2-GLO4, respectively. R-GLO represents the enzyme extracted from leaves of rice, and R-OxGLO4 represents the enzyme extracted from leaves of GLO4-overexpressed transgenic rice. This result is representative of five independent experiments.