Figure 5. GLO1 and GLO4 are the major contributors to GLO activities in rice.

(A) Semi-quantitative RT-PCR analysis of each GLO gene in the transgenic plants carrying the silencing construct. This result is representative of three independent experiments. (B) GLO enzyme activities in transgenic plants. RiGLO1, RiGLO3, RiGLO4 represent the specific GLO1, GLO3 and GLO4 RNA-silencing transgenic plants, respectively. The second leaf from the top was detached from plants at vegetative stage. Relative GLO activity was graphed based on the GLO activity of wild type (WT) as 1. The data represent means ±SD of 3 independent experiments.