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The YFP-tagged GLOs and CFP-tagged PTS1 fusion constructs were used in protoplast transient expression system to determine the subcellular localization of GLOs, the CFP-tagged PTS1 was used as the peroxisome marker. A1–A4: transfection of rice protoplasts; B1–B4: transfection of Arabidopsis protoplasts. This result is representative of three independent experiments.
