Table 1. Interactions of the GLOs determined by His-tag pull down assay.

Co-expressed GLOs	Activity recovery (%)
GLO1-his + GLO3	91.63%
GLO1-his + GLO4	96.79%
GLO3-his + GLO1	65.99%
GLO3-his + GLO4	35.65%
GLO4-his + GLO1	88.16%
GLO4-his + GLO3	68.47%
GLO5-his + GLO1	4.51%
GLO5-his + GLO3	5.71%
GLO5-his + GLO4	2.99%

GLO-his means the 6 amino acids on the C-terminus of GLO was mutated to a 6×his-tag. The interactions between every two GLOs are evaluated by calculating the activity recovery rate. The data are means of 3 independent experiments.