Table 1. Interactions of the GLOs determined by His-tag pull down assay.
Co-expressed GLOs | Activity recovery (%) |
GLO1-his + GLO3 | 91.63% |
GLO1-his + GLO4 | 96.79% |
GLO3-his + GLO1 | 65.99% |
GLO3-his + GLO4 | 35.65% |
GLO4-his + GLO1 | 88.16% |
GLO4-his + GLO3 | 68.47% |
GLO5-his + GLO1 | 4.51% |
GLO5-his + GLO3 | 5.71% |
GLO5-his + GLO4 | 2.99% |
GLO-his means the 6 amino acids on the C-terminus of GLO was mutated to a 6Ă—his-tag. The interactions between every two GLOs are evaluated by calculating the activity recovery rate. The data are means of 3 independent experiments.