Figure 2. pSTAT3/STAT3 and SOCS3 protein expression in He, Ob and DM myocytes in response to IL-6.

(A) Protein expression of STAT3, pSTAT3 and SOCS3 was assessed in satellite cells isolated from healthy (He) (n = 7), obese (Ob) (n = 7) and obese people with type 2 diabetes (DM) (n = 7) (Table 2). Satellite cells were differentiated into myocytes and treated with IL-6 (100 ng/ml) or control (PBS) for 30, 60 and 120 min (same protein samples as in Figure 1 B-D). (B) IL-6 induced phosphorylated STAT3 was related to total STAT3 levels and IL-6 treated samples were presented as fold change from PBS treated samples. IL-6 induction of pSTAT3 was compared between groups by two-way ANOVAs. (C) Baseline SOCS3 protein expression in the samples described in A was normalized to total protein expression (as determined by reactive brown staining) and compared between groups by two-way ANOVAs. (D) IL-6 induced SOCS3 protein expression was normalized to total protein expression and compared between groups by two-way ANOVAs. Data are presented as fold change (FC) between the control samples presented in A and C and samples treated with IL-6 (100 ng/ml). Data are mean ± SE. Groups were compared in pairs (i.e. He vs Ob, He vs DM and Ob vs DM) by two-way ANOVAs. Results from ANOVAs are marked in the figures with connecting capped arcs. *Results from Bonferroni post-tests, relative to He myocytes; *P<0.05, **P<0.01, ***P<0.001.