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. 2012 Jun 26;7(6):e38842. doi: 10.1371/journal.pone.0038842

Figure 6. Downregulation of ZEB2 expression induced cell cycle arrest at G1/S phase by regulating cell cycle-related genes.

Figure 6

A. The cell cycle distribution in siZEB2 treated and NC groups of U251 and U87 glioma cells by FACS Caliber cytometry. Histograms showing G1/S phase arrest and a decline in S phase in U251 and U87 cells after knocking down ZEB2 expression through targeted siRNA transfection. B and C. The protein levels of several key cell cycle regulators was analyzed by western blot. Reduced ZEB2 expression significantly inhibited cell cycle progression in U251 and U87 glioma cells. β-actin was used as a loading control. Bar graph shows the relative expression of protein among the groups. Data are presented were presented as mean ± SD for three independent experiments. *P<0.05, statistically significant difference.