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. 2012 Jun 26;7(6):e39458. doi: 10.1371/journal.pone.0039458

Figure 5. Agonist-selective phosphorylation of the sst3-sst2CT chimera.

Figure 5

(Top) Schematic representation of the rat sst3-sst2CT chimera indicating the phosphate acceptor sites S341/343, T353/354 and T356/359 within the carboxyl-terminal tail. (Bottom) HEK293 cells stably expressing the rat sst3-sst2ACT receptor were either not exposed or exposed to concentrations of 10−12 to 10−5 M SS-14, octreotide, pasireotide or somatoprim for 5 min. The levels of phosphorylated rsst3-sst2ACT receptors were then determined using anti-pS341/pS343 {3157}, anti-pT353/pT354 {0521} or anti-pT356/pT359 {0522}. Western blots shown are representative of three to five independent experiments for each condition. The positions of the molecular mass markers are indicated on the left (in kDa).