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. 2012 Jun 26;7(6):e39069. doi: 10.1371/journal.pone.0039069

Figure 4. Cell adhesion and migratory/invasive capacity of adherent and spheroid cells.

Figure 4

(A–C) Spheroid cells have lower capacities of both adhesions to FN (A) and to their homogeneous neighbors (B), as compared with their parental cells. The ICAM-1 expression in speroid cells was modestly down-regulated, while CXCR4 expression was up-regulated as compared to HCT116/HT29 monolayer cells respectively (C). (D and E) HCT116/HT29 spheroid cells showed a 3.8-fold (p<0.01) and 4-fold (p<0.001) increase in chemotactic potential at 24 h (D). More spheroid cells were obviously observed to invade Matrigel as compared to their parental counterparts (p<0.001 and p<0.05, respectively, E).